{"id":339,"date":"2023-09-11T20:03:15","date_gmt":"2023-09-11T20:03:15","guid":{"rendered":"http:\/\/www.precicolon.es\/?page_id=339"},"modified":"2023-10-14T16:36:24","modified_gmt":"2023-10-14T16:36:24","slug":"research-summary-fibhnjs","status":"publish","type":"page","link":"https:\/\/www.precicolon.es\/?page_id=339","title":{"rendered":"Research summary AP"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\"><strong>The Anatomic Pathology (AP) group<\/strong> was established fifteen years ago at the Hospital Fundaci\u00f3n Jim\u00e9nez D\u00edaz (FJD) in Madrid and is currently part of the Instituto de Investigaci\u00f3n Sanitaria Fundaci\u00f3n Jim\u00e9nez D\u00edaz (IIS-FJD). He has a consolidated career in the study of the molecular basis of the mechanisms of resistance generation to oncological therapies and in the translation to the clinic of strategies for the reversal of such resistance. Although an important part of its work has focused on breast cancer, the group has extensive experience in the study of colon cancer (as well as in some other tumor types). Our usual working procedures are based on human cell line models, which we then transfer to murine models to finally validate the results in collections of human clinical samples.<br>The research developed over the years allows us to provide expertise in the following areas:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Preclinical models:<\/strong> AP group has a long track record in the study of targeted therapies in cell lines and in the generation of models of acquired resistance to targeted drugs and chemotherapy, as reflected in several publications. We have also analyzed the role of the acquisition of an immature phenotype through the activation of the Hippo signaling pathway as an antibody resistance factor. This factor was identified from a multi-omic study (proteomic, genomic, microRNA). We have experience in the study of the tumor microenvironment and have developed cell co-culture models, combining the tumor cell with the stroma (CAF), immune cells (PBMCs or NK) and even 3D models. AP group also has experience in in vivo cancer models and have collaborated in the generation of PDX models.<\/li>\n\n\n\n<li><strong>Human samples:<\/strong> AP group pathologists in the group and in the department with extensive experience in cancer of different types, in the study of biomarkers, validation of laboratory methods and molecular characterization of tumors. <strong>Dr. Rojo<\/strong> is also the translational coordinator of GEICAM and has co-authored the international consensus on the determination of tumor infiltrating lymphocytes (TIL). S. Zazo and N. Carvajal have extensive experience in this same line of work and in the study of molecular techniques in cancer, including next-generation sequencing (NGS) and liquid biopsy. AP group has pioneered the development of ex vivo fresh tumor tissue cultures for molecular therapy analysis, which later evolved into studies of the immune system through the addition of peripheral blood mononuclear cells (PBMC), patient serum or natural killer (NK) cells. Due to its clinical background, the Anatomic Pathology group has, as part of its diagnostic routine, extensive experience in studies with various platforms for molecular tumor characterization (Prosigna, Oncotype, PAM50, NanoString, AVENIO, etc.).<\/li>\n\n\n\n<li><strong>Translational-clinical studies: <\/strong>Throughout these years AP group has generated, together with collaborating groups, several cohorts of patients with biological samples (paraffin-embedded tumors, fresh tumors, peripheral blood samples). groups, several cohorts of patients with biological samples (paraffin-embedded tumors, fresh tumors, peripheral blood samples). These studies have been oriented to the identification of biomarkers associated with the prediction of response to chemotherapy and targeted therapies, as reflected in their publications. In addition to retrospective series, AP group has led the generation of clinical studies with an important translational component, such as the KATIA trial (started in 2018), translational studies related to the GEICAM ConvertHER trial, as well as studies on phenotypic and mutational modification of breast cancer in disease progression, which have been presented at international congresses. In addition, IIS-FJD center is very active in clinical trials, including Phase I studies in which we perform molecular characterization of tumors by NGS and collect high-value samples.<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>The Anatomic Pathology (AP) group was established fifteen years ago at the Hospital Fundaci\u00f3n Jim\u00e9nez D\u00edaz (FJD) in Madrid and is currently part of the Instituto de Investigaci\u00f3n Sanitaria Fundaci\u00f3n Jim\u00e9nez D\u00edaz (IIS-FJD). He has a consolidated career in the &hellip; <a href=\"https:\/\/www.precicolon.es\/?page_id=339\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":324,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-339","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.precicolon.es\/index.php?rest_route=\/wp\/v2\/pages\/339","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.precicolon.es\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.precicolon.es\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.precicolon.es\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.precicolon.es\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=339"}],"version-history":[{"count":6,"href":"https:\/\/www.precicolon.es\/index.php?rest_route=\/wp\/v2\/pages\/339\/revisions"}],"predecessor-version":[{"id":695,"href":"https:\/\/www.precicolon.es\/index.php?rest_route=\/wp\/v2\/pages\/339\/revisions\/695"}],"up":[{"embeddable":true,"href":"https:\/\/www.precicolon.es\/index.php?rest_route=\/wp\/v2\/pages\/324"}],"wp:attachment":[{"href":"https:\/\/www.precicolon.es\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}